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High pressure 31P NMR spectroscopy on guanine nucleotides

Spoerner, Michael ; Karl, Matthias ; Lopes, Pedro ; Hoering, Marcus ; Loeffel, Karoline ; Nuehs, Andrea ; Adelsberger, Joseph ; Kremer, Werner ; Kalbitzer, Hans Robert



Abstract

The P-31 NMR pressure response of guanine nucleotides bound to proteins has been studied in the past for characterizing the pressure perturbation of conformational equilibria. The pressure response of the P-31 NMR chemical shifts of the phosphate groups of GMP, GDP, and GTP as well as the commonly used GTP analogs GppNHp, GppCH(2)p and GTP gamma S was measured in the absence and presence of ...

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