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Real-time imaging of photodynamic action in bacteria

Gollmer, Anita ; Felgenträger, Ariane ; Maisch, Tim ; Flors, Cristina



Abstract

Fluorescence imaging studies of the processes leading to photodynamic inactivation of bacteria have been limited due to the small size of microorganisms as well as by the faint fluorescence of most photosensitizers. A versatile method based on highly-sensitive fluorescence microscopy is presented which allows to study, in real time, the incorporation of photosensitizers inside S. aureus upon ...

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