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Application of laboratory and digital techniques for visual enhancement during the ultrastructural assessment of cilia

Schroeder, Josef A.



Abstract

Routine diagnostic electron microscopy of primary ciliary dyskinesia (PCD) is based on the findings of ultrastructural defects of axonemal components. Assessment of the typical abnormalities can be enhanced by improving the sample preservation status using tannic acid (TA) as additive in the biopsy fixation or processing steps. Another option is the implementation of computer-assisted image ...

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