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Mammalian cell surface display for monoclonal antibody-based FACS selection of viral envelope proteins

Bruun, Tim-Henrik ; Grassmann, Veronika ; Zimmer, Benjamin ; Asbach, Benedikt ; Peterhoff, David ; Kliche, Alexander ; Wagner, Ralf


The elicitation of broadly and efficiently neutralizing antibodies in humans by active immunization is still a major obstacle in the development of vaccines against pathogens such as the human immunodeficiency virus (HIV), influenza virus, hepatitis C virus or cytomegalovirus. Here, we describe a mammalian cell surface display and monoclonal antibody (mAb)-mediated panning technology that allows ...


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