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Mammalian cell surface display for monoclonal antibody-based FACS selection of viral envelope proteins

Bruun, Tim-Henrik, Grassmann, Veronika, Zimmer, Benjamin, Asbach, Benedikt, Peterhoff, David, Kliche, Alexander and Wagner, Ralf (2017) Mammalian cell surface display for monoclonal antibody-based FACS selection of viral envelope proteins. mAbs 9 (7), pp. 1052-1064.

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Other URL: http://doi.org/10.1080/19420862.2017.1364824


Abstract

The elicitation of broadly and efficiently neutralizing antibodies in humans by active immunization is still a major obstacle in the development of vaccines against pathogens such as the human immunodeficiency virus (HIV), influenza virus, hepatitis C virus or cytomegalovirus. Here, we describe a mammalian cell surface display and monoclonal antibody (mAb)-mediated panning technology that allows ...

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Item type:Article
Date:2017
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
10.1080/19420862.2017.1364824DOI
Keywords:IMMUNODEFICIENCY-VIRUS TYPE-1; BROADLY NEUTRALIZING ANTIBODIES; HIV-1 ENV TRIMERS; IG KNOCKIN MICE; SEQUENTIAL IMMUNIZATION; CONFORMATIONAL MASKING; GENE-EXPRESSION; INFECTION; BINDING; EPITOPE; HIV-1 envelope; neutralizing antibodies; mammalian cell display; panning; library
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:38849
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