Go to content
UR Home

Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation

Gödecke, Natascha ; Zha, Lisha ; Spencer, Shawal ; Behme, Sara ; Riemer, Pamela ; Rehli, Michael ; Hauser, Hansjörg ; Wirth, Dagmar



Abstract

Faithful expression of transgenes in cell cultures and mice is often challenged by locus dependent epigenetic silencing. We investigated silencing of Tet-controlled expression cassettes within the mouse ROSA26 locus. We observed pronounced DNA methylation of the Tet promoter concomitant with loss of expression in mES cells as well as in differentiated cells and transgenic animals. Strikingly, the ...

plus


Owner only: item control page
  1. Homepage UR

University Library

Publication Server

Contact:

Publishing: oa@ur.de
0941 943 -4239 or -69394

Dissertations: dissertationen@ur.de
0941 943 -3904

Research data: daten@ur.de
0941 943 -5707

Contact persons