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Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation

Gödecke, Natascha, Zha, Lisha, Spencer, Shawal, Behme, Sara, Riemer, Pamela, Rehli, Michael, Hauser, Hansjörg and Wirth, Dagmar (2017) Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation. Nucleic Acids Research 45 (16), e147-e147.

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Other URL: http://doi.org/10.1093/nar/gkx601

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Abstract

Faithful expression of transgenes in cell cultures and mice is often challenged by locus dependent epigenetic silencing. We investigated silencing of Tet-controlled expression cassettes within the mouse ROSA26 locus. We observed pronounced DNA methylation of the Tet promoter concomitant with loss of expression in mES cells as well as in differentiated cells and transgenic animals. Strikingly, the ...

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Item type:Article
Date:2017
Institutions:Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Identification Number:
ValueType
10.1093/nar/gkx601DOI
Keywords:EMBRYONIC STEM-CELLS; SITE-SPECIFIC INTEGRATION; DNA METHYLATION; MAMMALIAN-CELLS; DIRECT CONVERSION; ENDOGENOUS GENES; INDUCIBLE SYSTEM; VITAMIN-C; ES CELLS; TETRACYCLINE;
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:39166
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