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Synergistic effects of dendritic cell targeting and laser-microporation on enhancing epicutaneous skin vaccination efficacy

Machado, Yoan ; Duinkerken, Sanne ; Hoepflinger, Veronika ; Mayr, Melissa ; Korotchenko, Evgeniia ; Kurtaj, Almedina ; Pablos, Isabel ; Steiner, Markus ; Stoecklinger, Angelika ; Lübbers, Joyce ; Schmid, Maximillian ; Ritter, Uwe ; Scheiblhofer, Sandra ; Ablinger, Michael ; Wally, Verena ; Hochmann, Sarah ; Raninger, Anna M. ; Strunk, Dirk ; van Kooyk, Yvette ; Thalhamer, Josef ; Weiss, Richard


Due to its unique immunological properties, the skin is an attractive target tissue for allergen-specific immunotherapy. In our current work, we combined a dendritic cell targeting approach with epicutaneous immunization using an ablative fractional laser to generate defined micropores in the upper layers of the skin. By coupling the major birch pollen allergen Bet v 1 to mannan from S. ...


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