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Single Molecule Upconversion-Linked Immunosorbent Assay with Extended Dynamic Range for the Sensitive Detection of Diagnostic Biomarkers

Farka, Zdeněk , Mickert, Matthias J. , Hlaváček, Antonín, Skládal, Petr and Gorris, Hans H. (2017) Single Molecule Upconversion-Linked Immunosorbent Assay with Extended Dynamic Range for the Sensitive Detection of Diagnostic Biomarkers. Analytical Chemistry 89 (21), pp. 11825-11830.

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Other URL: http://doi.org/10.1021/acs.analchem.7b03542


Abstract

The ability to detect disease markers at the single molecule level promises the ultimate sensitivity in clinical diagnosis. Fluorescence-based single-molecule analysis, however, is limited by matrix interference and can only probe a very small detection volume, which is typically not suitable for real world analytical applications. We have developed a microtiter plate immunoassay for counting ...

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Item type:Article
Date:2017
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Identification Number:
ValueType
10.1021/acs.analchem.7b03542DOI
Keywords:PHOTON-UPCONVERTING NANOPARTICLES; PROSTATE-SPECIFIC ANTIGEN; BIOANALYTICAL APPLICATIONS; EUROPIUM NANOPARTICLES; QUANTITATIVE-ANALYSIS; IMMUNOASSAY; CHALLENGES; CANCER; PERSPECTIVES; PHASE;
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:39581
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