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Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry—Investigation of Pre-Analytic Stability

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Liebisch, Gerhard ; Ecker, Josef ; Roth, Sebastian ; Schweizer, Sabine ; Öttl, Veronika ; Schött, Hans-Frieder ; Yoon, Hongsup ; Haller, Dirk ; Holler, Ernst ; Burkhardt, Ralph ; Matysik, Silke
Date of publication of this fulltext: 03 May 2019 11:42


Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe-host communication. Here, we present a method for the quantification of SCFAs in fecal samples by liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The method ...


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