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Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry—Investigation of Pre-Analytic Stability

Liebisch, Gerhard, Ecker, Josef, Roth, Sebastian, Schweizer, Sabine, Öttl, Veronika, Schött, Hans-Frieder, Yoon, Hongsup, Haller, Dirk, Holler, Ernst, Burkhardt, Ralph and Matysik, Silke (2019) Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry—Investigation of Pre-Analytic Stability. Biomolecules 9 (4), p. 121.

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Date of publication of this fulltext: 03 May 2019 11:42

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Other URL: http://doi.org/10.3390/biom9040121


Abstract

Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe–host communication. Here, we present a method for the quantification of SCFAs in fecal samples by liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The method ...

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Item type:Article
Date:28 March 2019
Institutions:Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.3390/biom9040121DOI
Keywords:SCFA; mass spectrometry; feces; LC-MS/MS; method validation
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:40174
Owner only: item control page

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