Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry—Investigation of Pre-Analytic Stability

URN to cite this document:: urn:nbn:de:bvb:355-epub-401748
DOI to cite this document:: 10.5283/epub.40174

Liebisch, Gerhard

; Ecker, Josef

; Roth, Sebastian ; Schweizer, Sabine ; Öttl, Veronika ; Schött, Hans-Frieder ; Yoon, Hongsup ; Haller, Dirk ; Holler, Ernst ; Burkhardt, Ralph

; Matysik, Silke

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Date of publication of this fulltext: 03 May 2019 11:42

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Abstract

Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe-host communication. Here, we present a method for the quantification of SCFAs in fecal samples by liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The method ...