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Next Generation DNA-Seq and Differential RNA-Seq Allow Re-annotation of the Pyrococcus furiosus DSM 3638 Genome and Provide Insights Into Archaeal Antisense Transcription

Grünberger, Felix, Reichelt, Robert, Bunk, Boyke, Spröer, Cathrin, Overmann, Jörg, Rachel, Reinhard, Grohmann, Dina and Hausner, Winfried (2019) Next Generation DNA-Seq and Differential RNA-Seq Allow Re-annotation of the Pyrococcus furiosus DSM 3638 Genome and Provide Insights Into Archaeal Antisense Transcription. Frontiers in Microbiology 2019 (10), p. 1603.

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Date of publication of this fulltext: 25 Jul 2019 06:45

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Other URL: http://doi.org/10.3389/fmicb.2019.01603


Abstract

Pyrococcus furiosus DSM 3638 is a model organism for hyperthermophilic archaea with an optimal growth temperature near 100∘C. The genome was sequenced about 18 years ago. However, some publications suggest that in contrast to other Pyrococcus species, the genome of P. furiosus DSM 3638 is prone to genomic rearrangements. Therefore, we re-sequenced the genome using third generation sequencing ...

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Item type:Article
Date:12 July 2019
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Reinhard Rachel
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Dina Grohmann
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.3389/fmicb.2019.01603DOI
Keywords:archaea, Pyrococcus, RNA sequencing, Nanopore sequencing, PacBio sequencing, bidirectional transcription, antisense transcription
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:40570
Owner only: item control page

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