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Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

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Durst, Franziska C. ; Grujovic, Ana ; Ganser, Iris ; Hoffmann, Martin ; Ugocsai, Peter ; Klein, Christoph A. ; Czyż, Zbigniew T.
Date of publication of this fulltext: 31 Oct 2019 08:03


Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-amplified using a previously established whole transcriptome amplification (WTA) protocol. We ...


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