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Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

URN to cite this document:
urn:nbn:de:bvb:355-epub-408532
DOI to cite this document:
10.5283/epub.40853
Durst, Franziska C. ; Grujovic, Ana ; Ganser, Iris ; Hoffmann, Martin ; Ugocsai, Peter ; Klein, Christoph A. ; Czyż, Zbigniew T.
Date of publication of this fulltext: 31 Oct 2019 08:03



Abstract

Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-amplified using a previously established whole transcriptome amplification (WTA) protocol. We ...

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