Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

URN to cite this document:: urn:nbn:de:bvb:355-epub-408532
DOI to cite this document:: 10.5283/epub.40853

Durst, Franziska C. ; Grujovic, Ana

; Ganser, Iris ; Hoffmann, Martin ; Ugocsai, Peter ; Klein, Christoph A.

; Czyż, Zbigniew T.

Preview

License: Creative Commons Attribution 4.0
PDF
(1MB)

Date of publication of this fulltext: 31 Oct 2019 08:03

Alternative links to fulltext:DOI Publisher

Details

Preview

Indexed in

Export bibliographical data

Abstract

Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-amplified using a previously established whole transcriptome amplification (WTA) protocol. We ...