Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading

URN to cite this document:: urn:nbn:de:bvb:355-epub-413559
DOI to cite this document:: 10.5283/epub.41355

Nazet, Ute

; Schröder, Agnes ; Grässel, Susanne

; Muschter, Dominique

; Proff, Peter ; Kirschneck, Christian

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Date of publication of this fulltext: 10 Jan 2020 09:57

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Abstract

Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA) pathogenesis, but there is no information on reliable housekeeping genes available. Therefore the goal of this study was to identify a set of ...