Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro

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urn:nbn:de:bvb:355-epub-413566 Copy

Theinkom, Felix ; Singer, Larissa ; Cieplik, Fabian ; Cantzler, Sylvia ; Weilemann, Hannes ; Cantzler, Maximilian ; Hiller, Karl-Anton ; Maisch, Tim ; Zimmermann, Julia L.

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Date of publication of this fulltext: 10 Jan 2020 10:16

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Details

Item type:	Article
Journal or Publication Title:	PLOS ONE
Publisher:	Public Library of Science
Volume:	14
Number of Issue or Book Chapter:	11
Page Range:	e0223925
Date:	26 November 2019
Institutions:	Medicine > Lehrstuhl für Dermatologie und Venerologie
Projects:	Open Access Publizieren (DFG)
Identification Number:	Value Type 10.1371/journal.pone.0223925 DOI
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	41356

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Abstract

Nosocomial infections have become a serious threat in our times and are getting more difficult to handle due to increasing development of resistances in bacteria. In this light, cold atmospheric plasma (CAP), which is known to effectively inactivate microorganisms, may be a promising alternative for application in the fields of dentistry and dermatology. CAPs are partly ionised gases, which ...

Abstract

Nosocomial infections have become a serious threat in our times and are getting more difficult to handle due to increasing development of resistances in bacteria. In this light, cold atmospheric plasma (CAP), which is known to effectively inactivate microorganisms, may be a promising alternative for application in the fields of dentistry and dermatology. CAPs are partly ionised gases, which operate at low temperature and are composed of electrons, ions, excited atoms and molecules, reactive oxygen and nitrogen species. In this study, the effect of CAP generated from ambient air was investigated against Enterococcus faecalis, grown on agar plates or as biofilms cultured for up to 72 h. CAP reduced the colony forming units (CFU) on agar plates by > 7 log10 steps. Treatment of 24 h old biofilms of E. faecalis resulted in CFU-reductions by ≥ 3 log10 steps after CAP treatment for 5 min and by ≥ 5 log10 steps after CAP treatment for 10 min. In biofilm experiments, chlorhexidine (CHX) and UVC radiation served as positive controls and were only slightly more effective than CAP. There was no damage of cytoplasmic membranes upon CAP treatment as shown by spectrometric measurements for release of nucleic acids. Thus, membrane damage seems not to be the primary mechanism of action for CAP towards E. faecalis. Overall, CAP showed pronounced antimicrobial efficacy against E. faecalis on agar plates as well as in biofilms similar to positive controls CHX or UVC.

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