The CaMKII delta is a multifunctional serin-threonin-kinase and a potential therapeutical target in the clinical treatment of cardiovascular diseases. The CaMKII delta can be activated in many different ways, e.g. through Calcium/Calmodulin, through oxidation or through phosphorylation. The purpose of this study was to examine the reaction behavior of CaMKII delta in the context of autophosphorylation. The results of this study demonstrate among other things that there might be an interesting cross-talk between autophosphorylation and other activation mechanisms of CaMKII delta. This may be of great importance to understand the reaction behavior of CaMKII delta and for the development of new therapies.