| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Acta Anaesthesiologica Scandinavica | ||||
| Publisher: | WILEY-BLACKWELL | ||||
| Place of Publication: | HOBOKEN | ||||
| Volume: | 60 | ||||
| Number of Issue or Book Chapter: | 1 | ||||
| Page Range: | pp. 103-116 | ||||
| Date: | 2015 | ||||
| Institutions: | Medicine > Lehrstuhl für Anästhesiologie | ||||
| Identification Number: |
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| Keywords: | MYOCARDIAL INFARCT SIZE; NITRIC-OXIDE SYNTHASE; VASCULAR-SURGERY; LATE-PHASE; IN-VIVO; ISCHEMIA/REPERFUSION INJURY; VOLATILE ANESTHETICS; REPERFUSION INJURY; CONSCIOUS RABBITS; ISOFLURANE; | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 41789 |
Web of ScienceAbstract
Background: Late ischemic preconditioning is mediated via nuclear transcription factor signal transducer and activator of transcription 3 (STAT3). Pim-1 kinase reduces infarct size in cardiomyocytes and is regulated by STAT3. We tested the hypothesis that late desflurane-induced preconditioning (DES-SWOP) is mediated via STAT3 and Pim-1. Methods: After institutional approval, ...
Abstract
Background: Late ischemic preconditioning is mediated via nuclear transcription factor signal transducer and activator of transcription 3 (STAT3). Pim-1 kinase reduces infarct size in cardiomyocytes and is regulated by STAT3. We tested the hypothesis that late desflurane-induced preconditioning (DES-SWOP) is mediated via STAT3 and Pim-1. Methods: After institutional approval, pentobarbital-anesthetized male C57BL/6 mice were subjected to 45 min coronary artery occlusion (CAO) and 3 h reperfusion. Control animals received no additional intervention. Desflurane was administered 48 h before CAO either alone or in combination with the janus kinase/STAT3 inhibitor AG-490 (40 mu g/g i.p., 20 min before desflurane administration) or the Pim-1 kinase inhibitor II (PIM-Inh.II, 10 mu g/g i.p., 15 min before CAO). Infarct size (IS) and area at risk were determined with triphenyltetrazolium chloride and Evans blue, respectively. Additionally, cytosolic and nuclear fractions were separated at two different time points and expression of STAT3, phospho-STAT3(Ser727), phospho-STAT3(Tyr705), Pim-1, Bad and phospho-Bad(Ser112) were determined by Western Blot analysis. Data were analyzed with one-way or two-way ANOVA and post hoc Duncan test and are presented as mean +/- SEM. Results: IS was 47 +/- 2% (n = 7-8 per group) in control animals (CON). DES-SWOP reduced myocardial infarct size to 23 +/- 4%* (*P < 0.05 vs. CON). AG-490 alone did not affect myocardial infarct size (44 +/- 7%), but abolished DES-SWOP (44 +/- 4%). Blockade of Pim-1 did not affect the protection by DES-SWOP (34 +/- 4%*). Desflurane reduced cytosolic content and enhanced nuclear content of phospho-STAT(Ser727). After 48 h, desflurane enhanced Pim-1 activity, whereas Pim-1 expression remained unchanged. Conclusion: These data suggest that DES-SWOP is mediated by activation and nuclear translocation of STAT3. The impact of Pim-1 in DES-SWOP signaling remains unclear.
Metadata last modified: 17 Mar 2020 10:56
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