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Thiazole Orange Dimers in DNA: Fluorescent Base Substitutions with Hybridization Readout

Berndl, Sina ; Dimitrov, Stoichko D. ; Menacher, Florian ; Fiebig, Torsten ; Wagenknecht, Hans-Achim



Abstract

By using (S)-2-amino-1,3-propanediol as a linker, thiazole orange (TO) was incorporated in a dimeric form into DNA. The green fluorescence (=530nm) of the intrastrand TO dimer is quenched, whereas the interstrand TO dimer shows a characteristic redshifted orange emission (=585nm). Steady-state optical spectroscopic methods reveal that the TO dimer fluorescence is independent of the sequential ...

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