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Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

Sedlmeier, Andreas ; Hlaváček, Antonín ; Birner, Lucia ; Mickert, Matthias J. ; Muhr, Verena ; Hirsch, Thomas ; Corstjens, Paul L. A. M. ; Tanke, Hans J. ; Soukka, Tero ; Gorris, Hans H.


An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than ...


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