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Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Hlaváček, Antonín ; Farka, Zdeněk ; Hübner, Maria ; Horňáková, Veronika ; Němeček, Daniel ; Niessner, Reinhard ; Skládal, Petr ; Knopp, Dietmar ; Gorris, Hans H.



Abstract

Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) ...

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