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EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells

Galler, K. M. ; Widbiller, M. ; Buchalla, W. ; Eidt, A. ; Hiller, K. -A. ; Hoffer, P. C. ; Schmalz, G.



Abstract

Aim To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. Methodology Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H-2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was ...

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