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Quantification of oxysterols in human plasma and red blood cells by liquid chromatography high-resolution tandem mass spectrometry

Pataj, Zoltán ; Liebisch, Gerhard ; Schmitz, Gerd ; Matysik, Silke



Abstract

Oxysterols are important intermediates in numerous metabolic and catabolic pathways and their biological significance is also proven. The present paper describes a reliable and short liquid chromatography-high-resolution mass spectrometry method (LC-MS/HR-MS) for the quantification of 8 different oxysterols (24(S)-hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol, 4 ...

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