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CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

Schneider, Tim ; Hung, Lee-Hsueh ; Schreiner, Silke ; Starke, Stefan ; Eckhof, Heinrich ; Rossbach, Oliver ; Reich, Stefan ; Medenbach, Jan ; Bindereif, Albrecht



Abstract

Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no ...

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