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A reagentless enzymatic fluorescent biosensor for glucose based on upconverting glasses, as excitation source, and chemically modified glucose oxidase

del Barrio, Melisa ; Cases, Rafael ; Cebolla, Vicente ; Hirsch, Thomas ; de Marcos, Susana ; Wilhelm, Stefan ; Galbán, Javier



Abstract

Upon near-infrared excitation Tm3++Yb3+ doped fluorohafnate glasses present upconversion properties and emit visible light. This property permits to use these glasses (UCG) as excitation sources for fluorescent optical biosensors. Taking this into account, in this work a fluorescent biosensor for glucose determination is designed and evaluated. The biosensor combines the UCG and the fluorescence ...

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