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Determination of free clindamycin, flucloxacillin or tedizolid in plasma: Keep attention to physiological conditions when using ultrafiltration

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Dorn, Christoph ; Schießer, Selina ; Wulkersdorfer, Beatrix ; Hitzenbichler, Florian ; Kees, Martin G. ; Zeitlinger, Markus
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 19 Mar 2020 10:24


Pharmacokinetic/pharmacodynamic indices of antiinfective drugs should be referenced to free drug concentrations. In the present study, clindamycin, flucloxacillin or tedizolid have been determined in human plasma by HPLC-UV. The drugs were separated isocratically within 3-6 min at a C18 column using mixtures of phosphate buffer/acetonitrile of pH 7.1-7.2. Sample treatment for the determination of ...


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