siRNA Specificity: RNAi Mechanisms and Strategies to Reduce Off-Target Effects

URN to cite this document:: urn:nbn:de:bvb:355-epub-444873
DOI to cite this document:: 10.5283/epub.44487

Meister, Gunter

; Neumeier, Julia

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Date of publication of this fulltext: 15 Mar 2021 12:30

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Item type:	Article
Open Access Type:	Gold (with APC)
Journal or Publication Title:	Frontiers in Plant Science
Publisher:	Frontiers
Volume:	11
Number of Issue or Book Chapter:	526455
Page Range:	pp. 1-7
Date:	28 January 2021
Institutions:	Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister
Projects (Historical):	Open Access Publizieren (DFG), SFB 960
Keywords:	siRNAs, microRNAs, off-target effects, RISC, RNAi
Dewey Decimal Classification:	500 Science > 570 Life sciences
Status:	Submitted
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	44487

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Abstract

Short interfering RNAs (siRNAs) are processed from long double-stranded RNA (dsRNA), and a guide strand is selected and incorporated into the RNA-induced silencing complex (RISC). Within RISC, a member of the Argonaute protein family directly binds the guide strand and the siRNA guides RISC to fully complementary sites on-target RNAs, which are then sequence-specifically cleaved by the Argonaute ...