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Screening of Metal Complex – Amino Acid Side Chain Interactions by Potentiometric Titration

DOI to cite this document:
10.5283/epub.45
Kruppa, Michael ; Frank, Daniel ; Leffler-Schuster, Helga ; König, Burkhard
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Date of publication of this fulltext: 05 Aug 2009 13:21


Abstract

Reversible coordination of amino acid side chains to metal complexes is widely used in protein purification (IMAC technique), but available data on affinity and selectivity of such binding processes are limited. We use potentiometric titration of a series of metal complexes with vacant coordination sites in the presence of molecules resembling amino acid side chain functionalities to screen for ...

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