Accurate Lipid Quantification of Tissue Homogenates Requires Suitable Sample Concentration, Solvent Composition, and Homogenization Procedure—A Case Study in Murine Liver

URN to cite this document:: urn:nbn:de:bvb:355-epub-458043
DOI to cite this document:: 10.5283/epub.45804

Liebisch, Gerhard

; Höring, Marcus ; Krautbauer, Sabrina ; Hittl, Louisa ; Babl, Verena ; Sigruener, Alexander

; Burkhardt, Ralph

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Date of publication of this fulltext: 25 Jun 2021 16:36

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Abstract

Lipidomics aim to quantify lipid species in all kinds of samples, including tissues. To subject a fixed amount of sample to various workflows, tissue homogenates were frequently prepared at defined concentrations in water or by addition of organic solvents. Here, we investigated this first step of tissue lipidomics by quantitative flow injection analysis coupled to Fourier-Transform mass ...