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Two are better than one: HPoxBS - hairpin oxidative bisulfite sequencing

Giehr, Pascal ; Kyriakopoulos, Charalampos ; Lepikhov, Konstantin ; Wallner, Stefan ; Wolf, Verena ; Walter, Jörn



Abstract

The controlled and stepwise oxidation of 5mC to 5hmC, 5fC and 5caC by Tet enzymes is influencing the chemical and biological properties of cytosine. Besides direct effects on gene regulation, oxidised forms influence the dynamics of demethylation and re-methylation processes. So far, no combined methods exist which allow to precisely determine the strand specific localisation of cytosine ...

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