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Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Keller, Kate E. ; Bhattacharya, Sanjoy K. ; Borrás, Theresa ; Brunner, Thomas M. ; Chansangpetch, Sunee ; Clark, Abbott F. ; Dismuke, W. Michael ; Du, Yiqin ; Elliott, Michael H. ; Ethier, C. Ross ; Faralli, Jennifer A. ; Freddo, Thomas F. ; Fuchshofer, Rudolf ; Giovingo, Michael ; Gong, Haiyan ; Gonzalez, Pedro ; Huang, Alex ; Johnstone, Murray A. ; Kaufman, Paul L. ; Kelley, Mary J. ; Knepper, Paul A. ; Kopczynski, Casey C. ; Kuchtey, John G. ; Kuchtey, Rachel W. ; Kuehn, Markus H. ; Lieberman, Raquel L. ; Lin, Shan C. ; Liton, Paloma ; Liu, Yutao ; Lütjen-Drecoll, Elke ; Mao, Weiming ; Masis-Solano, Marisse ; McDonnell, Fiona ; McDowell, Colleen M. ; Overby, Darryl R. ; Pattabiraman, Padmanabhan P. ; Raghunathan, Vijay K. ; Rao, P. Vasanth ; Rhee, Douglas J. ; Chowdhury, Uttio Roy ; Russell, Paul ; Samples, John R. ; Schwartz, Donald ; Stubbs, Evan B. ; Tamm, Ernst R. ; Tan, James C. ; Toris, Carol B. ; Torrejon, Karen Y. ; Vranka, Janice A. ; Wirtz, Mary K. ; Yorio, Thomas ; Zhang, Jie ; Zode, Gulab S. ; Fautsch, Michael P. ; Peters, Donna M. ; Acott, Ted S. ; Stamer, W. Daniel



Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods ...

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