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Dentine matrix proteins: isolation and effects on human pulp cells

Widbiller, M. ; Eidt, A. ; Lindner, S. R. ; Hiller, K.-A. ; Schweikl, H. ; Buchalla, W. ; Galler, K. M.



Abstract

AimTo establish a simplified and efficient protocol for the isolation and concentration of matrix proteins from human dentine, and to assess the effects of extracted dentine matrix proteins (eDMP) on the behaviour of human pulp cells. MethodologyMatrix proteins were isolated from human dentine, purified, concentrated and characterized with protein and enzyme-linked immunosorbent assays (ELISA). ...

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