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Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

Ramos-Gomes, Fernanda ; Bode, Julia ; Sukhanova, Alyona ; Bozrova, Svetlana V. ; Saccomano, Mara ; Mitkovski, Miso ; Krueger, Julia Eva ; Wege, Anja K. ; Stuehmer, Walter ; Samokhvalov, Pavel S. ; Baty, Daniel ; Chames, Patrick ; Nabiev, Igor ; Alves, Frauke



Abstract

Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and ...

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