| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | International Journal of Hygiene and Environmental Health | ||||
| Publisher: | ELSEVIER GMBH, URBAN & FISCHER VERLAG | ||||
| Place of Publication: | JENA | ||||
| Volume: | 221 | ||||
| Number of Issue or Book Chapter: | 2 | ||||
| Page Range: | pp. 199-210 | ||||
| Date: | 2018 | ||||
| Institutions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene | ||||
| Identification Number: |
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| Keywords: | DRINKING-WATER; HOT-WATER; ENVIRONMENTAL-SAMPLES; LEGIONNAIRES-DISEASE; MASS-SPECTROMETRY; IDENTIFICATION; PNEUMOPHILA; COLONIZATION; PREVALENCE; HOSPITALS; Biodiversity; Drinking water quality; ISO 11731; MALDI biotyper | ||||
| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 47392 |
Web of ScienceAbstract
Therefore, in many countries it is mandatory to monitor warm water systems for their presence. The method of examination in Germany is regulated by guideline ISO 11731 and DIN EN ISO 11731-2, and the results are reported as concentration of Legionella spp. Only limited information is available on the presence of individual species of Legionellae in the examined systems, since most investigations ...
Abstract
Therefore, in many countries it is mandatory to monitor warm water systems for their presence. The method of examination in Germany is regulated by guideline ISO 11731 and DIN EN ISO 11731-2, and the results are reported as concentration of Legionella spp. Only limited information is available on the presence of individual species of Legionellae in the examined systems, since most investigations and research focus solely on Legionella pneuntophila as the most important human pathogen. In this study 76,220 samples obtained from 13,397 warm water systems originating from 24 different zip code districts covering an area of more than 71,0001km(2) in southern Germany were examined. This resulted in the identification of 47,924 Legionella isolates to the species level using a MALDI-TOF mass spectrometry-based method. Legionella species distribution was analyzed with respect to warm water system type, geographic region (defined as zip code district) and temperature during sample taking. Overall, 20.7% of the samples were found positive for Legionella species and 14 different species of Legionella were recovered. These were not equally present throughout the geographic area investigated, but instead an individual regional diversity of Legionella species was observed for the examined zip code districts. Although Legionella pnewnophila represented 84% of all contaminations found, depending on the geographical region its proportion varied substantially between 57.5% and 91.2%. The occurrence of other species was also of importance since they accounted for up to 42% of contaminations regionally, with Legionella londiniensis being most prominent representing up to 38.8% of recovered colonies. In addition, the influence of temperature on the individual species was disparate, but the temperature range between 50 degrees C and 59 degrees C was identified as the optimal condition for facilitating emergence of the majority of recovered Legionella species. The identification of Legionella to the species level by MALDI-TOF allowed for a more concise depiction of the regional distribution and the ecology of this genus, and may be of additional value when counter measures need to be initiated.
Metadata last modified: 28 Jul 2021 17:22
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