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Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
Hitzenbichler, Florian
, Bauernfeind, Stilla , Salzberger, Bernd , Schmidt, Barbara and Wenzel, Jürgen J.
(2021)
Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2.
Viruses 13 (4), p. 653.
Date of publication of this fulltext: 27 Aug 2021 11:47
Article
DOI to cite this document: 10.5283/epub.47901
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute ...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were 5.8x10(4) copies/mL, 85% (NS), 1.4x10(4), 79% (OS) and 4.3x10(3), 85% (TW). Concentration differences were significant between NS and TW (P = 0.019). Saliva (SA) was available from 21 patients (median 3.4x10(3)). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.
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Details
| Item type | Article | ||||
| Journal or Publication Title | Viruses | ||||
| Publisher: | MDPI | ||||
|---|---|---|---|---|---|
| Place of Publication: | BASEL | ||||
| Volume: | 13 | ||||
| Number of Issue or Book Chapter: | 4 | ||||
| Page Range: | p. 653 | ||||
| Date | 10 April 2021 | ||||
| Institutions | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene | ||||
| Identification Number |
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| Keywords | SARS-CoV-2; COVID-19; throat washing; nasopharyngeal swab; oropharyngeal swab; saliva; nucleic acid test; PCR; RT-qPCR; diagnostic sensitivity | ||||
| Dewey Decimal Classification | 600 Technology > 610 Medical sciences Medicine | ||||
| Status | Published | ||||
| Refereed | Yes, this version has been refereed | ||||
| Created at the University of Regensburg | Yes | ||||
| URN of the UB Regensburg | urn:nbn:de:bvb:355-epub-479012 | ||||
| Item ID | 47901 |
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