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High-resolution polymer high performance liquid chromatography: optimization of the saw tooth gradient profile for various stationary phases and separations on preparative scale

Durner, Bernhard ; Ehmann, Thomas ; Matysik, Frank-Michael


The recently introduced saw tooth gradient protocol for high-resolution polymer HPLC was further improved and optimized in terms of total runtime and separation performance. As a result, increased flow rates enabled drastically reduced runtimes in combination with enhanced peak resolutions. Moreover, the saw tooth gradient profile was further investigated using a saw tooth gradient with a ...


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