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Online mass spectrometry of CE (SDS)-separated proteins by two-dimensional capillary electrophoresis

Römer, Jennifer ; Montealegre, Cristina ; Schlecht, Johannes ; Kiessig, Steffen ; Moritz, Bernd ; Neusüß, Christian



Abstract

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the fundamental technique for protein separation by size. Applying this technology in capillary format, gaining high separation efficiency in a more automated way, is a key technology for size separation of proteins in the biopharmaceutical industry. However, unequivocal identification by online mass spectrometry (MS) is ...

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