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1H NMR spectroscopy quantifies visibility of lipoproteins, subclasses, and lipids at varied temperatures and pressures

Baumstark, Daniela ; Kremer, Werner ; Boettcher, Alfred ; Schreier, Christina ; Sander, Paul ; Schmitz, Gerd ; Kirchhoefer, Renate ; Huber, Fritz ; Kalbitzer, Hans Robert


NMR-based quantification of human lipoprotein (sub)classes is a powerful high-throughput method for medical diagnostics. We evaluated select proton NMR signals of serum lipoproteins for elucidating the physicochemical features and the absolute NMR visibility of their lipids. We separated human lipoproteins of different subclasses by ultracentrifugation and analyzed them by H-1 NMR spectroscopy at ...


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