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Efficient determination of axon number in the optic nerve: A stereological approach

Koschade, Sebastian E. ; Koch, Marcus A. ; Braunger, Barbara M. ; Tamm, Ernst R.



Abstract

Quantifying the number of axons in the optic nerve is of interest in many research questions. Here, we show that a stereological method allows simple, efficient, precise and unbiased determination of the total axon number in the murine optic nerve. Axons in semi-thin optic nerve cross sections from untreated eyes (n = 21) and eyes subjected to retinal damage by intravitreous NMDA injections (n = ...

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