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3D-Imaging of Whole Neuronal and Vascular Networks of the Human Dental Pulp via CLARITY and Light Sheet Microscopy

França, Cristiane Miranda ; Riggers, Rachelle ; Muschler, John L. ; Widbiller, Matthias ; Lococo, Peter Manning ; Diogenes, Anibal ; Bertassoni, Luiz Eduardo


Direct visualization of the spatial relationships of the dental pulp tissue at the whole-organ has remained challenging. CLARITY (Clear Lipid-exchanged Acrylamide Tissue hYdrogel) is a tissue clearing method that has enabled successful 3-dimensional (3D) imaging of intact tissues with high-resolution and preserved anatomic structures. We used CLARITY to study the whole human dental pulp with ...


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