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A sensitive and simple targeted proteomics approach to quantify transcription factor and membrane proteins of the unfolded protein response pathway in glioblastoma cells

Nguyen, Chi D. L. ; Malchow, Sebastian ; Reich, Stefan ; Steltgens, Sascha ; Shuvaev, Konstantin V. ; Loroch, Stefan ; Lorenz, Christin ; Sickmann, Albert ; Knobbe-Thomsen, Christiane B. ; Tews, Björn ; Medenbach, Jan ; Ahrends, Robert



Abstract

Many cellular events are driven by changes in protein expression, measurable by mass spectrometry or antibody-based assays. However, using conventional technology, the analysis of transcription factor or membrane receptor expression is often limited by an insufficient sensitivity and specificity. To overcome this limitation, we have developed a high-resolution targeted proteomics strategy, which ...

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