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Synthesis and characterisation of α-carboxynitrobenzyl photocagedl-aspartates for applications in time-resolved structural biology

Zaitsev-Doyle, John J. ; Puchert, Anke ; Pfeifer, Yannik ; Yan, Hao ; Yorke, Briony A. ; Müller-Werkmeister, Henrike M. ; Uetrecht, Charlotte ; Rehbein, Julia ; Huse, Nils ; Pearson, Arwen R. ; Sans, Marta



Abstract

We report a new synthetic route to a series of a-carboxynitrobenzyl photocaged L-aspartates for application in time-resolved structural biology. The resulting compounds were characterised in terms of UV/Vis absorption properties, aqueous solubility and stability, and photocleavage rates (tau = ms to ms) and quantum yields (phi = 0.05 to 0.14).


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