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Aptamer lateral flow assays for rapid and sensitive detection of cholera toxin

Frohnmeyer, Esther ; Tuschel, Nadine ; Sitz, Tobias ; Hermann, Cornelia ; Dahl, Gregor T. ; Schulz, Florian ; Baeumner, Antje J. ; Fischer, Markus


Aptamers are envisioned to serve as powerful synthetic substitutes to antibodies in a variety of bioanalytical assay formats. However, lateral flow assays (LFAs) remain dominated by antibody-based strategies. In this study, a LFA for the detection of cholera toxin as a model analyte is developed and optimized using a synthetic aptamer and a naturally occurring receptor as biorecognition elements ...


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