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Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells

Flores-Tornero, María ; Proost, Sebastian ; Mutwil, Marek ; Scutt, Charles P. ; Dresselhaus, Thomas ; Sprunck, Stefanie


Key messageA protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes.AbstractSexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate ...


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