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Computational Immune Monitoring Reveals Abnormal Double-Negative T Cells Present across Human Tumor Types

Greenplate, Allison R. ; McClanahan, Daniel D. ; Oberholtzer, Brian K. ; Doxie, Deon B. ; Roe, Caroline E. ; Diggins, Kirsten E. ; Leelatian, Nalin ; Rasmussen, Megan L. ; Kelley, Mark C. ; Gama, Vivian ; Siska, Peter J. ; Rathmell, Jeffrey C. ; Ferrell, P. Brent ; Johnson, Douglas B. ; Irish, Jonathan M.


Advances in single-cell biology have enabled measurements of >40 protein features on millions of immune cells within clinical samples. However, the data analysis steps following cell population identification are susceptible to bias, time-consuming, and challenging to compare across studies. Here, an ensemble of unsupervised tools was developed to evaluate four essential types of immune cell ...


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