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Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Kopczynski, Dominik ; Hentschel, Andreas ; Coman, Cristina ; Schebb, Nils Helge ; Hornemann, Thorsten ; Mashek, Douglas G. ; Hartung, Nicole M. ; Shevchuk, Olga ; Schött, Hans-Frieder ; Lorenz, Kristina ; Torta, Federico ; Burla, Bo ; Zahedi, René P. ; Sickmann, Albert ; Kreutz, Michael R. ; Ejsing, Christer S. ; Medenbach, Jan ; Ahrends, Robert


We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of similar to 150 and revealed that the insulin signaling is ...


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