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Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry

Farka, Zdeněk ; Mickert, Matthias J. ; Mikušová, Zuzana ; Hlaváček, Antonín ; Bouchalová, Pavla ; Xu, Wenshu ; Bouchal, Pavel ; Skládal, Petr ; Gorris, Hans H.



Abstract

Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate ...

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