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Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Farka, Zdeněk ; Mickert, Matthias J. ; Pastucha, Matěj ; Mikušová, Zuzana ; Skládal, Petr ; Gorris, Hans H.



Abstract

The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and ...

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