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2D and 3D immunogold localization on (epoxy) ultrathin sections with and without osmium tetroxide

Flechsler, Jennifer ; Heimerl, Thomas ; Pickl, Carolin ; Rachel, Reinhard ; Stierhof, York‐Dieter ; Klingl, Andreas


For nearly 50 years immunogold labeling on ultrathin sections has been successfully used for protein localization in laboratories worldwide. In theory and in practice, this method has undergone continual improvement over time. In this study, we carefully analyzed circulating protocols for postembedding labeling to find out if they are still valid under modern laboratory conditions, and in ...


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