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Improved CE(SDS)‐CZE‐MS method utilizing an 8‐port nanoliter valve

Römer, Jennifer ; Kiessig, Steffen ; Moritz, Bernd ; Neusüß, Christian


Capillary sieving electrophoresis utilizing SDS (CE(SDS)) is one of the most applied methods for the analysis of antibody (mAb) size heterogeneity in the biopharmaceutical industry. Inadequate peak identification of observed protein fragments is still a major issue. In a recent publication, we introduced an electrophoretic 2D system, enabling online mass spectrometric detection of generic CE(SDS) ...


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