Protein kinase C targeting of luminal (T-47D), luminal/HER2-positive (BT474), and triple negative (HCC1806) breast cancer cells in-vitro with AEB071 (Sotrastaurin) is efficient but mediated by subtype specific molecular effects

URN to cite this document:

urn:nbn:de:bvb:355-epub-519519 Copy

DOI to cite this document:

10.5283/epub.51951 Copy

Albert, Veruschka ; Piendl, Gerhard ; Yousseff, Dali ; Lammert, Hedwig ; Hummel, Michael ; Ortmann, Olaf ; Jagla, Wolfgang ; Gaumann, Andreas ; Wege, Anja K. ; Brockhoff, Gero

Preview

License: Creative Commons Attribution 4.0 PDF - Published Version (2MB)

Date of publication of this fulltext: 22 Mar 2022 05:45

---

Alternative links to fulltext:DOIPublisher

---

This publication is part of the DEAL contract with Springer.

---

Details

Item type:	Article
Open Access Type:	DEAL (Springer)
Journal or Publication Title:	Archives of Gynecology and Obstetrics
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
Date:	17 March 2022
Institutions:	Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde)
Identification Number:	Value Type 10.1007/s00404-022-06434-2 DOI
Keywords:	INHIBITOR AEB071; ALPHA EXPRESSION; PKC; PROMOTES; MODEL; Protein kinase C; AEB071; Sotrastaurin; Breast cancer
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Partially
Item ID:	51951

Preview

Indexed in

Web of Science

Export bibliographical data

ASCII CitationAtomBibTeXData Cite XMLDataCite XML OAIDublin CoreDublin Core (LZV)EndNoteHTML CitationJSONJSON LDMETSMODSOPENAIREOpenAPCRDF+N-TriplesRDF+N3RDF+XMLReferReference ManagerSimple MetadataXMLopenCostxMetaDissPlus

---

Abstract

Purpose Protein kinase C (PKC) plays a pivotal role in malignant cell proliferation, apoptosis, invasiveness and migration. However, its exploitation as therapeutic target in breast cancer has been merely explored. Here were evaluated the AEB071 (Sotrastaurin (TM)) treatment efficiency of breast cancer cell lines derived from estrogen receptor positive (T-47D), estrogen/HER2 receptor positive ...

Abstract

Purpose Protein kinase C (PKC) plays a pivotal role in malignant cell proliferation, apoptosis, invasiveness and migration. However, its exploitation as therapeutic target in breast cancer has been merely explored. Here were evaluated the AEB071 (Sotrastaurin (TM)) treatment efficiency of breast cancer cell lines derived from estrogen receptor positive (T-47D), estrogen/HER2 receptor positive (BT474), and triple negative (HCC1806) breast cancer cells under 2D (monolayer) and 3D (multicellular tumor spheroids) culture conditions. Additionally, spheroid cocultures of BC and N1 fibroblasts were analyzed. Methods We quantitatively assessed the proliferation capacity of breast cancer cells and fibroblasts as a function of AEB071 treatment using flow cytometry. The activities of PKC isoforms, substrates, and key molecules of the PKC signaling known to be involved in the regulation of tumor cell proliferation and cellular survival were additionally evaluated. Moreover, a multigene expression analysis (PanCancer Pathways assay) using the nanoString (TM) technology was applied. Results All breast cancer cell lines subjected to this study were sensitive to AEB071 treatment, whereby cell proliferation in 2D culture was considerably (BT474) or moderately (HCC1806) retarded in G0/G1 or in G2/M phase (T-47D) of the cell cycle. Regardless of the breast cancer subtype the efficiency of AEB071 treatment was significantly lower in the presence of N1 fibroblast cells. Subtype specific driver molecules, namely IL19, c-myb, and NGFR were mostly affected by the AEB071 treatment. Conclusion A combined targeting of PKC and a subtype specific driver molecule might complement specified breast cancer treatment.

---

---

Metadata last modified: 26 Jan 2023 15:23

Owner only: item control page

Download Statistics

Download Statistics

Download Statistics

Download Statistics

Downloads

Downloads per month over past year

Altmetric

Altmetric

Altmetric

Alternative Statistics (altmetrics)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)