Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

URN to cite this document:: urn:nbn:de:bvb:355-epub-525682
DOI to cite this document:: 10.5283/epub.52568

Willkomm, Sarah

; Jakob, Leonhard ; Kramm, Kevin ; Graus, Veronika ; Neumeier, Julia ; Meister, Gunter ; Grohmann, Dina

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Abstract

Human Argonaute 2 (hAgo2) constitutes the functional core of the RNA interference pathway. Guide RNAs direct hAgo2 to target mRNAs, which ultimately leads to hAgo2-mediated mRNA degradation or translational inhibition. Here, we combine site-specifically labeled hAgo2 with time-resolved single-molecule FRET measurements to monitor conformational states and dynamics of hAgo2 and hAgo2-RNA complexes ...