Extracellular Vesicles Derived from Osteogenic-Differentiated Human Bone Marrow-Derived Mesenchymal Cells Rescue Osteogenic Ability of Bone Marrow-Derived Mesenchymal Cells Impaired by Hypoxia

URN to cite this document:

urn:nbn:de:bvb:355-epub-549274 Copy

DOI to cite this document:

10.5283/epub.54927 Copy

Wang, Chenglong ; Stöckl, Sabine ; Pattappa, Girish ; Schulz, Daniela ; Hofmann, Korbinian ; Ilic, Jovana ; Reinders, Yvonne ; Bauer, Richard J. ; Sickmann, Albert ; Grässel, Susanne

License: Creative Commons Attribution 4.0 PDF - Published Version (6MB)

Date of publication of this fulltext: 26 Oct 2023 13:55

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Details

Item type:	Article
Open Access Type:	Gold (with APC)
Höhe Gebühr (aus OpenAPC):	2390.22
Institution der Zahlung:	Regensburg U
Journal or Publication Title:	Biomedicines
Publisher:	MDPI
Volume:	11
Number of Issue or Book Chapter:	10
Page Range:	p. 2804
Date:	16 October 2023
Institutions:	Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie
Projects (Historical):	Deutsche Gesellschaft für Orthopädie und Orthopädische Chirurgie, Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen, Bürgermeister von Berlin, Senatskanzlei Wissenschaft und Forschung
Identification Number:	Value Type 10.3390/biomedicines11102804 DOI
Keywords:	extracellular vesicles; BMSC; osteogenic differentiation; hypoxia; normoxia; ROS; antioxidants
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	54927

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Abstract (German)

In orthopedics, musculoskeletal disorders, i.e., non-union of bone fractures or osteoporosis, can have common histories and symptoms related to pathological hypoxic conditions induced by aging, trauma or metabolic disorders. Here, we observed that hypoxic conditions (2% O2) suppressed the osteogenic differentiation of human bone marrow-derived mesenchymal cells (hBMSC) in vitro and simultaneously ...

Abstract (German)

In orthopedics, musculoskeletal disorders, i.e., non-union of bone fractures or osteoporosis, can have common histories and symptoms related to pathological hypoxic conditions induced by aging, trauma or metabolic disorders. Here, we observed that hypoxic conditions (2% O2) suppressed the osteogenic differentiation of human bone marrow-derived mesenchymal cells (hBMSC) in vitro and simultaneously increased reactive oxygen species (ROS) production. We assumed that cellular origin and cargo of extracellular vesicles (EVs) affect the osteogenic differentiation capacity of hBMSCs cultured under different oxygen pressures. Proteomic analysis revealed that EVs isolated from osteogenic differentiated hBMSC cultured under hypoxia (hypo-osteo EVs) or under normoxia (norm-osteo EVs) contained distinct protein profiles. Extracellular matrix (ECM) components, antioxidants and pro-osteogenic proteins were decreased in hypo-osteo EVs. The proteomic analysis in our previous study revealed that under normoxic culture conditions, pro-osteogenic proteins and ECM components have higher concentrations in norm-osteo EVs than in EVs derived from naïve hBMSCs (norm-naïve EVs). When selected for further analysis, five anti-hypoxic proteins were significantly upregulated (response to hypoxia) in norm-osteo EVs. Three of them are characterized as antioxidant proteins. We performed qRT-PCR to verify the corresponding gene expression levels in the norm-osteo EVs’ and norm-naïve EVs’ parent cells cultured under normoxia. Moreover, we observed that norm-osteo EVs rescued the osteogenic ability of naïve hBMSCs cultured under hypoxia and reduced hypoxia-induced elevation of ROS production in osteogenic differentiated hBMSCs, presumably by inducing expression of anti-hypoxic/ antioxidant and pro-osteogenic genes.

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Metadata last modified: 20 Jan 2025 14:45

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