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An in vitro coculture approach to study the interplay between dental pulp cells and Streptococcus mutans

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Akamp, Tobias ; Rosendahl, Andreas ; Galler, Kerstin M. ; Wölflick, Melanie ; Buchalla, Wolfgang ; Widbiller, Matthias
Date of publication of this fulltext: 17 Nov 2023 09:23

This publication is part of the DEAL contract with Wiley.


Aim To develop a new coculture system that allows exposure of dental pulp cells (DPCs) to Streptococcus mutans and dentine matrix proteins (eDMP) to study cellular interactions in dentine caries. Methodology Dental pulp cells and S. mutans were cocultured with or without eDMP for 72 h. Cell proliferation and viability were assessed by cell counting and MTT assays, while bacterial growth and ...


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